Time (min) Flow Rate (mL/min) %A (Aqueous Modifier) %B (Organic Modifier)
0.01 1.00 95 5
1.00 1.00 5 95
2.50 1.00 5 95
2.60 1.00 95 5
3.50 1.00 95 5
Table 1: Generic gradient method used for the analysis of reference compounds and SHS15.
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