Variables Levels   Low Medium High Independent variables (factors)       X1; Flow rate: mL/min 0.6 0.8 1.0 X2; Injection volume: µL 10 15 20 X3; Detection wavelength: nm 265 270 275 X4; Initial gradient acetonitrile ratio: % v/v 60 70 80 X5; Acetonitrile ratio at four min of gradient run: % v/v 85 90 95 Coded levels of levels of X1, X2, X3, X4 and X5 -1 0 +1 Dependent variables (responses) Y1; Peak area of STP: mAU.s Y2; Peak area of HI443: mAU.s Y3; USP Tailing of STP Y4; USP Tailing of HI443

Table 1:Variables with their corresponding values in the experimental design.

Goto home»