Parameter HPLC method UV method ACO PCM TZN ACO PCM TZN Retention time (min) 1.89 2.87 3.34 - - - Working λmax (nm) - - - 277 248 323 Linearity (µg/mL) 5-30 10-60 2-12 5-30 2-16 2-30 LOD (µg/mL) 0.019 0.224 0.111 0.81 0.21 0.29 LOQ (µg/mL) 0.588 0.678 0.338 1.4 0.717 0.582 Precision(%RSD*) Intra-day precision 0.08 0.17 0.023 0.023 0.014 0.027 Inter-day precision 0.35 0.54 0.047 0.047 0.023 0.029 Theoretical plate/meter 20186.48 37466.62 42965.18 - - - Asymmetry 0.9 1.2 1.2 - - - Resolution 6.914 3.175 - - - Sandell’s sensitivity - - - 0.029 0.011 0.027 Slope 18802 99382 65853 0.033 0.092 0.0189 Intercept 8890 48412 3314 0.008 0.005 0.0295 Regression coefficient (r2) 0.999 0.999 0.999 0.999 0.999 0.999 *Average of three readings

Table 2: Validation parameter of HPLC and UV Method

Goto home»