ara-GTP concentration (pmol/106 cells)
  −30°C −80°C
  Day1 Day3 Day6 Day6 Day13 1 month 2 months
MeOH / 28% ammonia water (7/3, v/v)
Mean 18.08 19.65 14.60 18.05 20.24 19.12 17.57
SD 0.46 1.22 0.88 2.91 2.41 1.22 4.30
CV% 2.5 6.2 6.0 16.1 11.9 6.4 24.5
% Diff −9.6 −1.7 −27.0 −9.8 1.2 −4.4 −12.2
MeOH / 250 mM ammonium carbonate solution (7/3, v/v)
Mean 17.00 13.01 12.06 14.81 13.56 9.65 11.00
SD 1.11 2.30 3.08 1.75 3.09 3.19 3.64
CV% 6.5 17.7 25.6 11.8 22.8 33.0 33.1
% Diff −15.0 −35.0 −39.7 −25.9 −51.7 −45.0  
% Diff, versus nominal concentration (20 pmol/106 cells)
Table 4: Long Term Stability of Ara-GTP in Cell Extracts Treated with Methanol/Ammonia Water or Methanol/Ammonium Carbonate Solution.
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