| Primers (GeneBank accession no.) |
Sequence |
Size (bp) |
| Type I collagen
(NM_007742.3) |
F: 5’-CACTGCCCTCCTGACGCAT-3’
R: 5’-CACACAGCCGTGCCATTG-3’ |
137 |
| Bone sialoprotein
(NM_008318.3) |
F: 5’-CCAGGACTGCCGAAAGGAAG-3’
R: 5’-CCCCGTTTTCTTCAGAATCCTCTG -3’ |
144 |
| Osteocalcin
(NM_031368.4) |
F: 5’-CGGCCCTGAGTCTGACAAA -3’
R: 5’-GCCGGAGTCTGTTCACTACCTT -3’ |
67 |
| Ob-Ra
(NM_001122899.1) |
F: 5’-ACACTGTTAATTTCACACCAGAG -3’
R: 5’-AGTCATTCAAACCATAGTTTAGG -3’ |
236 |
| Ob-Rb
(NM_146146.2) |
F: 5’-ACACTGTTAATTTCACACCAGAG -3’
R: 5’-TGGATAAACCCTTGCTCTTCA -3’ |
445 |
| GAPDH
(NR_003623.1) |
F: 5’-CCATCACCATCTTCCAGGAG -3’
R: 5’-GCATGGACTGTGGTCATGAG -3’ |
303 |
