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Figure 1: Analyses of sAŽPP< and AŽLevels in Human Plasma and Culture Media from PBMC. Blood was collected into tubes containing lithium heparin (Li), K2EDTA (ED), sodium heparin (Na) and buffered sodium citrate (Cit) anticoagulants. AŽ1-40 and AŽ1-42 in plasma (A) and PBMC culture media (B) were quantitated by ELISA and expressed as pg/mL. sAŽPP< in plasma and PBMC was quantitated by ELISA and expressed as ng/mL (C). Measurements for the culture media were normalized based on cell counts. Statistical significance between groups was determined by one-way ANOVA followed by Student T-tests or Bonferroni’s multiple comparison, n=5 per anticoagulant. AŽ1- 42 in plasma: one way ANOVA, P<0.03, F=4; Student T-Test, Li versus Cit and Na versus Cit, P<0.05. sAŽPP< in plasma: one way ANOVA, P<0.0001, F=26; Bonferroni’s multi-comparison test, Li versus ED, Li versus Cit, ED versus Na and Na versus Cit, P<0.05. No statistically significant differences were found by one-way ANOVA of AŽ1-40 in plasma, AŽ1-40 and AŽ1-42 in culture media or sAŽPP< in culture media.
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