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Figure 1: ChAT expression measured in lysates of caudate, nbM, frontal cortex and hippocampus obtained from AD and age-matched control brains. Panel A. ChAT activity is expressed in nmol/mg protein/h. Statistical differences were found for ChAT activity in the cortex of AD brains compared to control [*p < 0.05]; differences in ChAT activity measured in control and AD hippocampal extract did not achieve statistical significance [p=0.07]. Panel B. Steady-state levels of ChAT mRNA analysed densitometrically, with values expressed as a ratio of β-actin mRNA from the same sample. Panel C. Representative ethidium bromide-stained gels showing amplification of M-ChAT and β-actin transcripts from RNA extracted from control or AD tissue. Lane 1 in both panels is a 100-bp DNA ladder with the brightest marker at 600 bp. Samples were taken from frontal cortex (FCtx), cerebellum (CB), hippocampus (H), caudate (C) and nucleus basalis of Meynert (NbM) of control (lanes 2 to 6) and AD (lanes 7 to 15) brains. Panel D. Steady-state levels of M-ChAT mRNA were analysed densitometrically with values expressed as a ratio of β-actin mRNA from the same sample. Panel E. Steady-state levels of M-ChAT mRNA as a ratio of β-actin mRNA from the same sample for NbM graphed relative to age. In each Panel, data are presented as mean±SEM.
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