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Figure 2: Pattern of NOS expression in tissue lysates of caudate, nbM, cortex and hippocampus obtained from AD and age-matched control brains. Panel A. NOS activity is expressed in nmol/mg protein/h and each bar represents the mean±SEM. There was no statistically significant difference in NOS activity between control and AD tissue in any of the brain regions examined. Panel B. Representative data showing RT-PCR amplification products of specific NOS isoform transcripts from RNA extracted from control and AD tissue. Primer sets were used for nNOS, eNOS, iNOS and ß-actin. Lane 1 in all panels is a 100-bp DNA ladder with the brightest marker at 600 bp. Samples were taken from frontal cortex (FCtx), cerebellum (CB), hippocampus (H), caudate (C) and nucleus basalis of Meynert (NbM) of control (lanes 2 to 6) and AD (lanes 7 to 15) brain. Steady-state levels of mRNA for nNOS (Panel C.) and eNOS (Panel D.) were analysed densitometrically with values expressed as a ratio of ß-actin mRNA from the same sample; data are presented as mean ± SEM. Statistical differences were found for eNOS levels in the cortex and hippocampus of AD brains compared to control, denoted by *p < 0.05.
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