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Figure 4: Trophic factor measurements in postmortem brains. Frontal lobe aqueous homogenates from subjects with Braak Stage (B) 0-1 (controls), B3-4 (moderate AD), or B6 (late AD) pathology (N=8/group) were used to measure (A) NGF, (B) BDNF, (C) PDGF-AA, (D) glial cell derived neurotrophic factor (GDNF), (E) hepatocyte growth factor (HGF), and (F) basic fibroblast growth factor (FGF) by direct binding ELISA. Immunoreactivity was detected with HRP-conjugated secondary antibody and Amplex Red soluble fluorophor. Fluorescence was measured (Ex 579 nm/Em 595 nm) in a Spectromax M5, and results were normalized to sample protein content. Data were analyzed by one-way repeated measures ANOVA with post hoc Fisher significance tests.
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