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Figure 1: Quantification and evaluation of the tight junctions were done in electron micrographs by counting all TJ’s in epithelial duodenal cells starting at the apical region and continuing towards the base of the cell in a 5000 nm section (Control dog in Figure 1a, EM x 30,000 ). Electromicrographs were taken from each animal and the number of abnormal TJ’s identified (Figures 1b, c). Figure 1B shows an abnormal zonula ocludens (short arrows) along separation of the adjacent cytoplasmic membranes (*) in an exposed dog (EM x 80,000). Figure 1C shows intact gap junctions (arrows) adjacent to areas with discontinuity of the junctions (*) (EM x 80,000).
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