PCR diagnosis

Negative group

Time of mortality (P.I)

28 h

30 h

36 h

44 h

45 h

Single step PCR (1s5 - 1a16 primers)

-

-

-

+

+

+

Single step PCR (IK1 - IK2 primers)

-

-

-

-

-

+

Nested PCR

(IK1-IK2 → IK3 - IK4 primers)

-

+

+

+

+

+

Table 3: Detection of WSSV by PCR from the tissues of shrimps (P. monodon) challenged by intramuscular (I.M.) injection of WSSV inoculum from frozen cold stored (5th month) shrimp sample.
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