Sample Identity |
Negative control |
Liver in L-15 medium |
ISAV 101 in liver homogenate |
ISAV 102 in liver homogenate |
ISAV 105 in liver homogenate |
ISAV 103
in L-15 medium |
ISAV 104
in L-15 medium |
Positive control (ISAV 106 in L-15 medium) |
Sample Codes |
Lab’s own |
01, 0B, 0D, 012, 0M, 018 |
0A, 0G, 0E, 015, 0T, 0K |
08, 011, 0C, 0L, 0Q, 016 |
0H, 05, 07, 019, 017, 013 |
04, 06, 02, 0R, 0J, 0P |
09, 0F, 03, 0N, 014, 0S |
ISAV |
Testing Lab |
|
|
|
|
|
|
|
|
Lab A |
0 |
42.97±0.511 (3/6)2 |
41.02±0.49 |
31.14±15.37(5/6) |
29.9±0.91 |
35.41±0.33 |
32.24±0.32 |
25.13± 0.17 |
Lab B |
0 |
0 |
35.98±0.34 |
33.67±0.68 |
31.8±0.53 |
32.46±0.98 |
30.62±0.78 |
24.14±0.41 |
Lab C |
0 |
0 |
37.06±0.99 |
34.2±0.33 |
28.41±1.4 |
32.0±0.23 |
28.94±0.26 |
22.34±0.19 |
Lab D |
0 |
0 |
39.15±0.69 |
35.62±0.62 |
30.32± 1.3 |
34.26±1.0 |
30.91±0.4 |
24.16±0.89 |
Lab E |
0 |
0 |
35.84±0.56 |
33.97±0.39 |
30.81±1.8 |
33.95±0.56 |
30.74±0.4 |
23.82±1.1 |
Lab F |
0 |
0 |
30.9 (1/6) |
31.4±1.0 |
24.9±0.99 |
29.5±0.98 |
26.18±0.77 |
19.5±0.28 |
Lab G |
0 |
0 |
0 |
32.0± 0.4 (2/6) |
28.89±1.1 |
31.41±0.37 (2/6) |
31.04±0.99 |
25.98±1.93 |
Lab H |
No report |
No report |
No report |
No report |
No report |
No report |
No report |
No report |
Lab I |
0 |
0 |
38.85±0.62 |
36.18±0.86 |
28.13±0.62 |
34.39±0.79 |
31.28±0.62 |
23.72±0.78 |
Lab J |
0 |
0 |
37.82±1.3 (5/6) |
35.22±1.61 |
28.04±1.18 |
33.3±1.45 |
30.14±1.4 |
24.63±0.98 |
Lab K |
0 |
0 |
40.87±1.1 |
36.85±0.77 |
30.0 ± 0.59 |
35.11±1.03 |
31.78±0.64 |
24.09±0.44 |
Lab L |
0 |
0 |
0 |
31.11±0.66 |
26.59 ±1.18 |
29.39±0.27 |
26.58±52 |
20.58± 0.58 |
Lab M |
0 |
0 |
29.64 ±2.17 (3/6) |
29.06 ±1.69 |
27.05 ± 3.64 |
28.96 ±0.83 |
27.49 ±0.45 |
21.05±1.1 |
Lab N (AB) |
40 |
39.9±0.2 |
36.39±1.2 |
33.57±0.45 |
24.2 ± 0.59 |
31.23±1.4 |
28.08±1.43 |
22.1±0.11 |
Lab O (Denmark) |
0 |
0 |
0 |
38.12±0.60 |
29.04±0.85 |
34.41±0.60 |
31.76±0.87 |
25.2±1.27 |
OIE Ref Lab, AVC |
0 |
0 |
34.98±0.39 |
31.97±0.38 |
31.27±0.64 (3/6) |
32.55± 0.58 |
29.63±0.38 |
22.7±0.55 |
Mean Ct value ± SD1 for assays performed on 6 different days. Where a sample had no Ct value (i.e., Ct = 0), the number of days in which it had a Ct value (Ct = >0) out
of the six days it was tested is given in brackets2.
In Blue: denotes mean Ct values that are ≥3 more than expected.
In green: denotes unexpected false negative(s).
In magenta: denotes sensitivity of real-time RT-PCR for ISAV is below that of most laboratories.
In red: denotes poor performing laboratory based on having more than two days (a) with mean Ct values that are ≥3 more than expected, and/or (b) with unexpected
false negative(s), and/or (c) unable to detect a virus titer of 103 TCID50/ml.
General Observations:
1. Cut off Ct values range between≥40 and ≥35 = negative.
2. No lab had a false positive result.
3. Lowest amount of virus titer detected by most laboratories is 103 TCID50/ml.
4. The best performing laboratories are: Lab B and Lab E. |
