Sample Identity

Negative control

Liver in L-15 medium

ISAV 101 in liver homogenate

ISAV 102 in liver homogenate

ISAV 105 in liver homogenate

ISAV 103
in L-15 medium

ISAV 104
in L-15 medium

Positive control (ISAV 106 in L-15 medium)

Sample Codes

Lab’s own

01, 0B, 0D, 012, 0M, 018

0A, 0G, 0E, 015, 0T, 0K

08, 011, 0C, 0L, 0Q, 016

0H, 05, 07, 019, 017, 013

04, 06, 02, 0R, 0J, 0P

09, 0F, 03, 0N, 014, 0S

ISAV

Testing Lab

 

 

 

 

 

 

 

 

OIE Ref Lab, A

0

0

34.98±0.39

31.97±0.38

31.27±0.64 (3/6)

32.55± 0.58

29.63±0.38

22.7±0.55

Lab B

0

0

0

32.0± 0.4 (2/6)

28.89±1.1

31.41±0.37 (2/6)

31.04±0.99

25.98±1.93

Lab C

0

0

35.98±0.34

33.67±0.68

31.8±0.53

32.46±0.98

30.62±0.78

24.14±0.41

Lab D

0

0

30.9 (1/6)

31.4±1.0

24.9±0.99

29.5±0.98

26.18±0.77

19.5±0.28

Lab E

0

0

40.87±1.1

36.85±0.77

30.0 ± 0.59

35.11±1.03

31.78±0.64

24.09±0.44

Lab F

0

0

39.15±0.69

35.62±0.62

30.32± 1.3

34.26±1.0

30.91±0.4

24.16±0.89

Lab G

0

0

0

31.11±0.66

26.59 ±1.18

29.39±0.27

26.58±52

20.58± 0.58

Lab H

No report

No report

No report

No report

No report

No report

No report

No report

Lab I

0

0

35.84±0.56

33.97±0.39

30.81±1.8

33.95±0.56

30.74±0.4

23.82±1.1

Lab J

0

0

29.64 ±2.17 (3/6)

29.06 ±1.69

27.05 ± 3.64

28.96 ±0.83

27.49 ±0.45

21.05±1.1

Lab K

0

0

0

38.12±0.60

29.04±0.85

34.41±0.60

31.76±0.87

25.2±1.27

Lab L

0

0

38.85±0.62

36.18±0.86

28.13±0.62

34.39±0.79

31.28±0.62

23.72±0.78

Lab M

0

0

37.06±0.99

34.2±0.33

28.41±1.4

32.0±0.23

28.94±0.26

22.34±0.19

Lab N

0

0

37.82±1.3 (5/6)

35.22±1.61

28.04±1.18

33.3±1.45

30.14±1.4

24.63±0.98

Lab O

40

39.9±0.2

36.39±1.2

33.57±0.45

24.2 ± 0.59

31.23±1.4

28.08±1.43

22.1±0.11

Lab V

0

42.97±0.511 (3/6)2

41.02±0.49

31.14±15.37(5/6)

29.9±0.91

35.41±0.33

32.24±0.32

25.13± 0.17

In Blue: denotes mean Ct values that are ≥3 more than expected.
In green: denotes unexpected false negative(s).
In magenta: denotes sensitivity of real-time RT-PCR for ISAV is below that of most laboratories.
In red: denotes poor performing laboratory based on having more than two days (a) with mean Ct values that are ≥3 more than expected, and/or (b) with unexpected false
negative(s)
, and/or (c) unable to detect a virus titer of 103 TCID50/ml.
Table 3: Overall performance (inter-lab and inter-assay) of different laboratories using real-time RT-PCR protocols for amplification of ISAV segment 8 Mean Ct value SD1 for assays performed on 6 different days. Where a sample had no Ct value (i.e., Ct = 0), the number of days in which it had a Ct value (Ct = >0) out of the six days it was tested is given in brackets2.
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