Items Control diet (On DM basis, %) Feed ingredients   Herring fish meal, 72% CP 10 Soybean meal, 44% CP 42 Yellow corn 24 Rice bran 10 Wheat bran 10 Sunflower oil 3.0 Vitamins1 0.5 Minerals premix2 0.5 Total 100 Chemical composition (%)   Dry matter 88.98 Crude protein 30.11 Ether extract 8.19 Crude fiber 7.50 Total ash 7.71 Nitrogen free extract3 46.49 Calculated energy value   GE (kcal/kg)4 4437 DE (kcal/kg)5 3328 P/E,mg/kcal6 90.47 1Mineral premix consisted of (mg kg-1 premix): 2600 mg Mn, 600 mg Cu, 6000 mg Fe, 4600 mg Zn, 50 mg Se, 100 mg Iu, 50 mg Co, 100,000 mg choline chloride, up to 1 kg carrier (Local market). 2Vitamin premix consisted of (mg kg-1 premix): 1,200, 000 IU Vitamin A, 400,000 IU Vitamin D3, 3000 IU Vitamin E, 1200 mg K3, 5400 mg C, 200 mg 2, 200 mg B1, 3360 mg B2, 7200 mg B3, 9000 mg B5, 2400 mg B6, 600 mg B9, 4 mg B12 (Local market). 3Nitrogen free extract concluded by difference 4GE (Gross energy) was calculated according to NRC [12] by using factors of 5.65, 9.45 and 4.22 K cal per gram of protein, lipid and carbohydrate, respectively . 5DE (Digestible energy) was calculated by applying the coefficient of 0.75 to convert gross energy to digestible energy according to Hepher et al. [39]. 6P/E (protein energy ratio)=crude protein×1000/digestible energy/100 g, according to Hepher et al. [39]. **Diets: D1 (control): without supplements, D2: 0.1% DIGESTON-1, D3: 0.3% DIGESTON-1, D4: 0.5% DIGESTON-1 and D5: 0.7% DIGESTON-1. **Values of diets content were within the range suggested for tilapia by Jauncey and Ross [40] and NRC [12].

Table 2: Composition and proximate analysis of the experimental diet.

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