Ingredient g/100g    
Control (0) 5 10 20
White fish meal 41 41 41 41
Casein 20 15 10 -
Flounder skin meal (FSM) - 5 10 20
L-ascorbic acid 0.02 0.02 0.02 0.02
Wheat flour 22.58 23.28 23.98 25.28
Feed oil (squid liver oil) 8.4 7.7 7 5.7
α-potato starch 3 3 3 2
Vitamin premix1) (vitamin C free) 2 2 2 2
Mineral Premix2) 2 2 2 1
Choline Chloride 1 1 1 1
Total 100 100 100 100
Vitamin C in diets 212.41 197.16 204.67 203.12
*Proximate analysis        
Protein 46.71 47.21 47.11 46.11
Lipid 9.21 9.51 10.11 10.81
Ash 8.21 8.61 8.71 8.71
1 Vitamin premix (mg/g mixture): retinol acetate, 0.81 mg; cholecalciferol, 0.012 mg; vitamin E, 22.5 mg; vitamin K3, 2.5 mg, thiamine, 5.5 mg; riboflavin, 10 mg; pyridoxine, 6 mg; niacin, 37.5 mg; folic acid, 2 mg; biotin 0.05 mg; inositol 50 mg. All ingredients were diluted with alpha-cellulose to 1g.
2 Mineral premix (mg/g mixture): Mn, 3.2 mg; Zn, 3.2 mg; Fe, 3.0 mg; Cu, 0.36 mg; MgSO4, 100 mg; KCl (47%), 60 mg; Al(OH)3, 1.06 mg; Ca(IO3)2, 0.475 mg; CoSO4, 0.475 mg. All ingredients were diluted with alpha-cellulose to 1g.
*Dry matter
Table 1: Ingredient and proximate composition of experimental diets with various levels of FSM.
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