content of whole body in black rockfish

	FSM substitution level (%)
	Control (0)	5	10	20
Phosphoserine	0.77 ± 0.03^b	0.84 ± 0.01^c	0.78 ± 0.02^b	0.62 ± 0.02^a
Taurine	3.31 ± 0.04^a	3.27 ± 0.17^a	3.41 ± 0.04^a	3.57 ± 0.09^b
Aspartic acid	3.31 ± 0.02^d	2.51 ± 0.02^c	1.61 ± 0.04^b	1.35 ± 0.02^a
Hydroxyproline	1.68 ± 0.05^b	1.23 ± 0.27^a	1.89 ± 0.13^b	1.70 ± 0.18^b
Threonine	3.18 ± 0.04^a	2.26 ± 0.06^b	1.54 ± 0.05^c	1.13 ± 0.02^d
Serine	2.19 ± 0.02^d	1.84 ± 0.09^c	1.39 ± 0.04^b	0.91 ± 0.02^a
Asparagine	0.88 ± 0.06^b	0.30 ± 0.02^a	0.30 ± 0.01^a	0.31 ± 0.00^a
Glutamic acid	5.46 ± 0.10^d	3.91 ± 0.05^c	2.57 ± 0.07^b	2.36 ± 0.05^a
Proline	1.95 ± 0.04^d	1.51 ± 0.10^c	1.19 ± 0.03^b	0.75 ± 0.03^a
Glycine	2.75 ± 0.03^c	2.97 ± 0.11^d	2.57 ± 0.09^b	2.38 ± 0.01^a
Alanine	5.95 ± 0.05^d	4.92 ± 0.08^c	3.79 ± 0.13^b	3.02 ± 0.01^a
Citrulline	0.34 ± 0.03^d	0.22 ± 0.04^c	0.11 ± 0.01^b	0.05 ± 0.00^a
Valine	3.10 ± 0.02^d	2.07 ± 0.05^c	1.53 ± 0.05^b	0.99 ± 0.02^a
Methionine	0.86 ± 0.02^d	0.48 ± 0.01^c	0.41 ± 0.02^b	0.32 ± 0.02^a
Isoleucine	2.46 ± 0.01^d	1.62 ± 0.02^c	1.10 ± 0.04^b	0.73 ± 0.01^a
Leucine	7.33 ± 0.01^d	4.33 ± 0.03^c	3.34 ± 0.13^b	2.54 ± 0.01^a
Tyrosine	1.35 ± 0.06^b	1.62 ± 0.06^c	1.44 ± 0.06^b	0.99 ± 0.03^a
Phenylalanine	2.84 ± 0.01^d	1.61 ± 0.02^c	1.33 ± 0.03^b	0.95 ± 0.01^a
β-aminoisobutyric acid	1.06 ± 0.07^b	0.53 ± 0.02^a	0.61 ± 0.12^a	0.55 ± 0.03^a
γ-amino-n-butyric acid	0.03 ± 0.01^b	0.01 ± 0.00^a	0.01 ± 0.00^a	0.02 ± 0.00^ab
Histidine	0.95 ± 0.01^d	0.56 ± 0.02^c	0.45 ± 0.02^b	0.20 ± 0.01^a
1-methylhistidine	0.06 ± 0.00^b	0.03 ± 0.01^a	0.02 ± 0.00^a	0.01 ± 0.01^a
Carnosine	0.21 ± 0.01^c	0.13 ± 0.03^a	0.14 ± 0.01^a	0.07 ± 0.02^a
Anserine	0.29 ± 0.06^ns	0.29 ± 0.05	0.31 ± 0.07	0.32 ± 0.01
Tryptopan	0.51 ± 0.09^b	0.22 ± 0.08^a	0.17 ± 0.07^a	0.09 ± 0.04^a
Hydroxylysine	0.11 ± 0.01^b	0.05 ± 0.01^a	0.04 ± 0.01^a	0.05 ± 0.02^a
Ornitine	0.19 ± 0.01^c	0.09 ± 0.01^b	0.06 ± 0.01^a	0.06 ± 0.00^a
Lysine	2.99 ± 0.07^d	0.77 ± 0.03^c	0.49 ± 0.02^b	0.33 ± 0.02^a
Ammonia	2.20 ± 0.03^c	1.86 ± 0.16^a	1.42 ± 0.03^a	1.55 ± 0.02^b
Ethanolamine	0.36 ± 0.08^ns	0.40 ± 0.03	0.34 ± 0.03	0.38 ± 0.04
Arginine	8.71 ± 0.12^d	5.84 ± 0.18^c	5.25 ± 0.16^b	3.57 ± 0.05^a
Total	67.37 ± 0.26^d	48.31 ± 0.99^c	39.61 ± 1.29^b	31.88 ± 0.22^a

Data are mean ± SD. Values with different superscripts are significantly different (P<0.05).
^ns Not significant.

Table 5: Free amino acid content of whole body in black rockfish (S. schlegeli) fed the test diets with various levels of FSM for 8 weeks (g/100 g).

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