Digestive Enzyme activity  LF AF Feeding regime
 MF13 LF-AF-13 LF-AF-18 LF-MF-13
Specific amylase activity
(mg maltose mg protein-1 h-1)
0.09 ± 0.01d 0.15 ± 0.02c 0.13 ± 0.01c 0.16 ± 0.02c 0.23 ± 0.01a 0.19 ± 0.01b
Chitinase
(mg NAG g-1 wet weight h-1)
44.31 ± 0.21a 5.91 ± 0.85e 19.56 ± 3.02b 8.73 ± 0.2d 17.73 ± 0.84c 20.4 ± 2.33b
Chitinobiase
(mg NAG g-1 wet weight h-1)
44.73 ± 0.36a 5.13 ± 0.46d 17.64 ± 0.15c 5.22 ± 0.20d 17.68 ± 2.61c 21.33 ± 0.49b
Protease
(mUnits mg protein-1 min-1)
569.72 ± 52.0d 682.5 ± 57.4c 1219.32 ± 20b 432.66 ± 28.8e 661.64 ± 28.8c 1261 ± 52.0a
Trypsin
(mUnits mg protein-1 min-1)
38.62 ± 4.14d 49.31 ± 6.64c 60.77 ± 7.86b 38.0 ± 2.49d 57.15 ± 2.60c 74.86 ± 5.78a
Chymotrypsin
(mUnits mg protein-1 min-1)
519.21 ± 10b 172.14 ± 5e 485.81 ± 10c 363.64 ± 20d 471.30 ± 15c 552.43 ± 9a
Carboxypeptidase A
(∆ Abs g-1 tissue min-1)
2.66 ± 0.4f 12.46 ± 1.42c 24.32 ± 1.41a 18.37 ± 2.18b 9.70 ± 0.93d 6.68 ± 0.48e
Carboxypeptidase B
(∆ Abs g-1 tissue min-1)
1.16 ± 0.26d 1.4 ± 0.11d 5.08 ± 0.98b 7.74 ± 0.12a 5.14 ± 1.37b 3.24 ± 0.57c
Specific lipase activity (mUnits mg protein-1) 109.34 ± 6.73e 131.64 ± 8.1d 179.53 ± 10.04b 150.27 ± 8c 167.31 ± 7.1b 227.93 ± 17a
Table 2: Digestive enzyme profiles of Catla catla cultured in six different feeding regimes. Mean (n= 3) followed by different letters in the same row are significantly (p<0.05) different.
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