Water sample location   Hormone WRAS (n=3) exchange rate Makeup influent (A) Pre-unit processes (B) Post-unit processes (C) Cortisol High 555.8 ± 186.6 575.6 ± 121.6 545.1 ± 214.3 Low 319.7 ± 181.0 448.6 ± 191.6 448.6 ± 176.4 Testosterone High 123.7 ± 7.313 518.7 ± 118.0 443.7 ± 86.32 Low 124.0 ± 45.24 768.4 ± 88.88 758.5 ± 155.5 11-ketotestosterone High 4.783 ± 0.390 194.5 ± 21.19 127.9 ± 11.08 Low 4.526 ± 1.008 183.0 ± 17.73 124.7 ± 11.90 Progesterone High 1134 ± 63.43 1422 ± 121.6 1699 ± 420.1 Low 1339 ± 491.0 1551 ± 263.5 1742 ± 286.4 Estradiol High 39.55 ± 6.341 168.7 ± 61.80 168.8 ± 66.25 Low 38.92 ± 25.06 223.5 ± 28.53 239.8 ± 20.69

Table 2: Hormone levels in water collected from makeup influent (A) and pre- (B) and post- (C) unit processes sample locations. Values reported are mean (± standard error) hormone concentrations (pg/250mL) for water samples in each treatment group, adjusted for dilution factor and resuspension volume. Different superscript letters within each hormone summary represent statistical trends (0.10 ≥ p ≥ 0.05) or significant differences (p<0.05) between quantified hormone levels (Table 3 summarizes relationships with p ≤ 0.10).

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