No.
Primer name
PCR Codition
Product Size (bp)
Annealing
Mg Conc.
dNTPs
Cycle Number
( OC)
(mM)
(mM)
(cycles)
1
DTLSH 7
60
2
200
40
131
2
DTLSH 8
60
2
200
40
174
3
DTLSH 9
60
2
200
40
210
4
DTLSH 12
60
2
200
40
193 / 205
Table 2:
PCR reduction component and primer qualities.
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