Parameters Control Mt0.05 Mt0.25 P value
Motility (%) 4.0b ± 1.8 11.2a ± 5.6 12.5a ±3.5 0.004
Progressive (%) 1b ± 0 2a± 1.1 2aa ± 0.6 0.048
Path velocity (VAP) (µm/s) 42.8 ± 4.3 48.7 ± 8.1 48.9 ± 11.6 0.3
Prog. velocity (VSL) (µm/s) 34.8 ± 3.6 38.3 ± 4.4 39.4 ± 10.1 0.4
Track speed (VCL) (µm/s) 67.1 ± 9.8 68.4 ± 12.4 71.7 ± 19 0.8
Lateral amplitude (ALH) (µm) 5.6 ± 2.4 6.2 ± 2.4 7.3 ± 2.9 0.5
Beat frequency (BCF) (Hz) 29.5 ± 7.5 32.0 ± 4.8 29.9 ± 4.2 0.7
Straightness (STR) (%) 79.2 ± 14 79.5 ± 7.4 80.3 ± 5.4 0.9
Linearity (LIN) (%) 52.7 ± 6.8 54.0 ± 9.6 57.2 ± 4.6 0.5
a,bValues with different superscripts in a row differ significantly (P<0.05).
Table 2: Sperm kinetic parameters measured by CASA for male C. macrocephalus with different levels of melatonin (P<0.05) (Mean ± S.E.)
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