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| Figure 3: Tissue expression profile of PajCHS. End-point RT-PCR products of PajCHS were separated on 1.5% agarose gel and stained with ethidium bromide (inverted image). Amplification of an 18SrRNA product was used as a positive control. The larger size of genomic DNA PCR products (Ge) confirmed the PCR reaction was not contaminated with genomic DNA Abbreviations: M, molecular size marker; Ep, epidermis; Hp, hepatopancreas; Gi, gill; Go, gonad; Ex, extensor muscle; Fl, flexor muscle; Ht, heart; In, intestine; Ag, abdominal ganglion; Tg, thoracic ganglion; Br, brain; Sg, sinus gland. |
