| Ingredients |
Diet6 No. |
| D1 |
D2 |
D3 |
D4 |
D5 |
| Fish meal |
100 |
100 |
100 |
100 |
100 |
| Soybean meal |
380 |
375 |
380 |
375 |
380 |
| Yellow corn |
200 |
200 |
200 |
200 |
200 |
| Wheat bran |
120 |
100 |
100 |
100 |
100 |
| Rice bran |
150 |
150 |
120 |
150 |
120 |
| Oil mixture1 |
30 |
30 |
30 |
30 |
30 |
| Vitamin and mineral premix2 |
20 |
20 |
20 |
20 |
20 |
| Ulva lactuca |
0 |
25 |
50 |
0 |
0 |
| Pterocladiacapillacea |
0 |
0 |
0 |
25 |
50 |
| Total |
1000 |
1000 |
1000 |
1000 |
1000 |
| Proximate composition and energy content (% dry matter basis) |
| Dry matter |
90.35 |
90.54 |
90.41 |
90.45 |
90.38 |
| Crude protein |
29.55 |
29.48 |
29.62 |
29.42 |
29.50 |
| Crude lipid |
8.97 |
8.87 |
8.76 |
8.66 |
8.57 |
| Crude fiber |
5.43 |
5.64 |
5.78 |
5.86 |
6.05 |
| Crude ash |
7.54 |
7.64 |
7.87 |
8.12 |
8.20 |
| Nitrogen free extract |
48.51 |
48.37 |
47.97 |
47.94 |
47.68 |
| GE (kcal/kg diet)3 |
456.4 |
454.5 |
452.6 |
450.5 |
448.9 |
| DE (kcal/kg diet)4 |
342.3 |
340.9 |
339.5 |
337.9 |
336.7 |
| P/E ratio (mg CP/kcal DE)5 |
86.4 |
86.5 |
87.2 |
87.1 |
87.6 |
|
| 1Mixture of sunflower oil and linseed oil with a ratio of 1:1. |
| 2Commercial vitamin (Super Vit, Arab Veterinary Industrial Co., Jordan). 15,000 IU vitamin A, 0.7 g vitamin C (Stay C®, 35% active), 15,000 IU vitamin D3, 2 mg vitamin E, 2.5 mg vitamin B2, 2 mg vitamin K3, 10 mg nicotineamide, 3 mg vitamin B6, 5 mg vitamin B12, 2 mg vitamin B1, 2 mg folic acid, 5.5 mg Ca-D-pantothenate and mineral premix (Eco Vit, Egyptian Veterinary Produced and Feed a Additives Co., Demyatta, Egypt). 200 g calcium, 90 g phosphate, 40 g sodium, 2.5 g copper, 48 g magnesium, 3.6 g manganese, 23.5 g zinc, 8 g iron, 450 mg cobalt, 200 mg iodine and 20 mg selenium. |
| 3GE (Gross energy) was calculated according to NRC [19] by using factors of 5.65, 9.45 and 4.22 K cal per gram of protein, lipid and carbohydrate, respectively. |
| 4DE (Digestible energy) was calculated by applying the coefficient of 0.75 to convert gross energy to digestible energy according to Hepher et al. [20]. |
| 5P/E (protein energy ratio)=crude protein×1000/digestible energy/100 g, according to Hepher et al. [20]. |
| 6Diets: D1 (control): without supplements, D2: 2.5% Ulvalactuca, D3:5% Ulvalactuca and D4: 2.5% Pterocladiacapillacea and D5 5% Pterocladiacapillacea. |
| **Values of diets content were within the range suggested for tilapia by Jauncey and Ross [21] and NRC [19]. |
| Table 2: Formulation and chemical proximate composition of the experimental diets (g/kg dry weight basis). |
