Phylogenetic group Band no. Closest relative (obtained from BLAST search) Identity (%) Accession no. Relative abundance (RA, %)
Auto-HD Auto-LD Allo-HD Allo-LD Feed
Actinobacteria 9 Uncultured Actinobacterium (AY795724.2) 95 EU585899 0.5 0.1 2.4 1.4
  41 Uncultured Corynebacterium sp. (EU029393.1) 100 EU585931 3.5 4.1 6.7 8.5
  11 Uncultured Rubrobacterales bacterium (EF662655) 95 EU585901 3.6 1.6 2.2 1.6
Proteobacteria 7 Acinetobactersp. (EU260356.1) 100 EU585896 2.5
  43 Uncultured Acinetobacter sp. (EU704874.1) 99 EU585929 1.2
  17 Uncultured Eubacterium sp. (AM422244.1) 98 EU585907 2.6
  18 Uncultured Eubacterium sp. (AM422244.1) 98 EU585908 1.4
  37 Uncultured Eubacterium sp. (AM422244.1) 99 EU585928 2.7 3.6
  15 Uncultured gamma Proteobacterium (DQ676291.1) 98 EU585905 1.3
  16 Uncultured gamma Proteobacterium (DQ676291.1) 98 EU585906 1.7 2.9
  34 Uncultured Proteobacterium (EF664750.1) 98 EU585925 11.2
Firmicutes 12 Clostridium bifermentans (EU887825.1) 98 EU887825 2.6 12.7 7.9 5.1 3.1
  19 Clostridium bifermentans (EU887825.1) 100 EU585909 10.4
  20 Clostridium bifermentans (EU887825.1) 98 EU585910 0.5 1.4 1.9 9.0
  22 Clostridium paraputrificum (AY442815.1) 98 EU585913 1.2
  29 Exiguobacterium sp. ( EF101987.1) 100 EU585903 1.7 1.0
  40 Lactobacillales bacterium (EU728748.1) 99 EU585930 3.0 5.8 2.8
  3 Lactobacillus panis (EF412978.1) 100 EU585895 1.2 3.7 0.9
  42 Lactococcus garvieae (EU727199.1) 99 EU585927 24.6 33.1 16.0 19.4 4.7
  36 Streptococcus infantarius (AJ439568) 100 EU585923 2.0 3.0
  2 Uncultured Firmicutes bacterium (AB451793.1) 99 EU585891 10.0 6.1 15.4 12.8
Fusobacteria 6 Anaerobic bacterium G13(AB064328) 96 EU585894 5.6 8.5
  1 Cetobacterium somerae (AB353124.1) 100 EU585890 24.2 2.2 15.9 2.2 4.4
  5 Fusobacteria bacterium (AY579753.1) 97 EU585893 2.7 2.6 4.4 0.5
  4 Uncultured microorganism (EU181077.1) 99 EU585892 7.6 7.9 1.7 10.5
Cyanobacteria 30 Uncultured bacterium (AB254251.1) 98 EU585922 4.3 8.5 2.5 4.9 4.0
  31 Uncultured bacterium (AB254251.1) 100 EU585924 1.2 0.8
Unclassified_
Bacteria
27 Vaccinium corymbosum (DQ445025.1) 94 EU585919 2.5
  35 Uncultured Actinobacterium (DQ676371.1) 99 EU585920 1.7
  10 Uncultured Cyanobacterium (EU661291.1) 100 EU585900 3.6 6.0 1.8 3.2 2.7
  14 Uncultured Cyanobacterium (EU661291.1) 98 EU585902 2.7 4.8 2.6 3.1 14.4
  U1       1.8 3.3
  U6       3.5
  U8       4.4 5.5 5.7
  U9       1.1 1.9
  U11       0.8
Auto-HD: autochthonous gut microbiota of gibel carp cultivated at the high stocking density; Auto-LD: autochthonous gut microbiota of gibel carp cultivated at the low stocking density; Allo-HD: allochthonous gut microbiota of gibel carp cultivated at the High stocking density; Allo-LD: allochthonous gut microbiota of gibel carp cultivated at the low stocking density; Feed: microbiota of the diet †Relative abundance (RA, %) was represented by the percentage of a specific band density to the total band density
Table 2: Representative of autochthonous and allochthonous bacteria or clones and their relative abundance (RA, %) in DGGE fingerprint of gibel carp cultivated in high – and low density ponds, Eastern China.
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