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Figure 3: SEM images demonstrating the morphological changes in the anterior intestine of rainbow trout exposed to the different bacterial inoculums in the ex vivo challenge. A: control fed fish intestine exposed to PBS, B: Control fed fish intestine exposed to V. anguillarum; C: Control fed fish intestine exposed P. acidilactici; D: P. acidilactici fed fish exposed to V. anguillarum+P. acidilactici. Key: * irregular microvilli, IR irregular enterocyte formations, arrows = spaces/gaps forming between enterocytes (arrows) exposing intracellular tight junctions. Scale bars 10μm. |