Days SAA Hepcidin Precerebellin IgM IgT Lysozyme IL-1β
Group II, 0.2% β-glucan          
Day 0 -1.88[0.49;0.61] -2.22[0.40; 0,77] -1.09[0.68; 2.13] -1.02[0.78; 1.56] 1.05[0.84; 1.30] 1.79[1.28; 2.50] -9.25[0.11; 0.11]
Day14 2.54[1.77;3.64] 1.74[0.82; 3.71] 1.30[1.08; 1.56] 1.02[0.86; 1.22] 1.18[0.94; 1.47] -1.01[0.85; 1.25] -32.85[0,03; -0.03]
Day 28 -1.30[0.65;-1.07] 4.50[2.28; 8.89] 1.22[1.06; 1.40] 1.26[1.07; 1.48] 1.36[1.06; 1.75] -1.56[0.60; 0.70] -6.22[0.16; 0.16]
Day 42 -1.75[0.51;0.69] -1.37[0.57; 1.67] -1.52[0.61; 0.74] 1.01[0.88; 1.16] 1.19[1.05; 1.35] -1.65[0.55; 0.71] 1.53[0.86; 2.72]
Day 56 -1.47[0.57;1.00] -1.88[0.47; 0.75] -1.10[0.78; 1.15] 1.70[1.52; 1.90] 3.00[2.46; 3.66] 2.11[1.84; 2.42] -19.08[0.05; 0.05]
Group III, 2% β-glucan          
Day 0 -1.15[0.66;2.03] 5.52[1.86; 16.37] 1.36[1.00; 1.85] 1.54[1.22; 1.94] 1.02[0.81; 1.29] 1.59[1.10; 2.30] -1.02[0.54; -0.21]
Day14 -2.58[0.37;0.41] 1.04[0.66; 1.63] 1.00[0.90; 1.12] -1.07[0.83; 1.11] -1.27[0.67; 1.05] -1.68[0.54; 0.70] -19.27[0.05; -0.08]
Day 28 1.37[0.87;2.17] 3.14[1.64; 6.03] 1.07[0.90; 1.28] -1.01[0.83; 1.32] 1.18[0.99; 1.41] -2.41[0.39; 0.45] -10.86[0.09; 0.10]
Day 42 2.55[1.24;5.24] 1.03[0.57; 1.86] -1.13[0.78; 1.05] -1.20[0.78; 0.92] -1.33[0.69; 0.83] 1.21[1.03; 1.42] 2.11[1.59; 2.80]
Day 56 -1.78[0.52;0.63] -5.79 * [0.17; 0.18] -1.86[0.52; 0.56] -1.19[1.05; 1.35] 1.64[1.24; 2.17] 1.43[1.15; 1.78] -120.50[0.01; 0.08]
Group IV, 5% β-glucan          
Day 0 -2.41[0.40;0.43] -1.47[0.54; 1.54] -1.26[0.68; 1.02] 1.31[1.08; 1.60] -1.04[0.81; 1.28] -1.08[0.74; 1.43] 3.34[0.38; 28.95]
Day14 2.05[2.05;2.05] 2.00[6.59; 0.38] 1.25[1.11; 1.41] 1.61[1.03; 1.31] 1.34[1.11; 1.61] 1.48[1.27; 1.73] -10.09[0.10; 0.10]
Day 28 12.69 ***[8.75;18.41] 1.69[1.95; 0.90] 1.93[1.62; 2.29] -1.01[0.88; 1.14] 1.21[0.98; 1.49] 1.75[1.38; 2.22] -7.99[0.12; 0.13]
Day 42 2.05[;187.7] -1.60[0.39; 0.24] -1.06[0.76; -1.39] -1.09[0.78; 1.18] -1.68[0.56; 0.64] -1.33[0.61; 1.30] 1.53[1.15; 2.03]
Day 56 6.12[3.09;12.12] 3.27[2.50; 1.42] 1.77[1.28; 2.45] 1.65[1.40; 1.94] 2.27[1.95; 2.64] 2.04[1.16; 3.60] -52.74[0.02; 0.02]
Table 2: Expression of immune genes in the liver of rainbow trout. Data is presented as a fold increase or decrease of genes in groups fed with β-glucan compared tothe control group (fed β-glucan-free feed). Positive values indicate up-regulation and negative values indicate down-regulation. Confidence intervals are presented belowin parentheses. Significant differences are in bold. *: p<0.05, ***: p<0.001. The significant changes of hepcidin and SAA are presented graphically in Figure. 3. Trends(although significant) are highlighted in grey.
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