| |
AFLP1 |
AFLP2 |
AFLP3 |
| Restriction enzymes |
EcoRI – MseI |
EcoRI – MseI |
EcoRI – TaqI |
| Selective PCR * |
9 |
6 |
1 |
| Post-amplification restriction enzymes |
— |
— |
RsaI - HinfI - MboI
MseI - NlaIII - AluI |
| Sampling years |
2003–2004 |
|
2008–2009 |
|
2008–2009 |
|
| |
n |
Loci |
n |
Loci |
n |
Loci |
| Site D |
— |
— |
7 |
322 (141) |
22 |
152 (77) |
| Site F |
10 |
750 (385) |
9 |
330 (164) |
18 |
154 (75) |
| Site G |
— |
— |
8 |
317 (154) |
20 |
159 (81) |
| Site M |
6 |
759 (301) |
8 |
315 (148) |
19 |
156 (75) |
| Site Y |
5 |
641 (195) |
11 |
329 (159) |
23 |
157 (75) |
| Total LSP |
21 |
842 (476) |
43 |
349 (214) |
102 |
160 (99) |
| Lake M2 |
— |
— |
— |
— |
7 |
160 (42) |
| Lake O2 |
— |
— |
— |
— |
7 |
174 (86) |
| All lakes |
21 |
842 (476) |
43 |
349 (214) |
116 |
187 (136) |
| *Selective combinations used for AFLP 1 (EcoANN×MseCNN) AC×AG, AC×TC, GC×AG, AC×GC, CG×GC, CG×AG, GG×TT, AG×CC, GC×CC; AFLP 2 (EcoANN×MseCNN) AC×TC, CG×GC, AG×CC, GC×TT, AC×TT, AG×AG; AFLP 3 EcoACG×TaqCTA |
|
