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Figure 1: PCR products for amplification using primers in second PCR (1.1) and sequencing chromatogram (1.2). In Figure 1.1, Lane 1is DNA marker (100 bp) and Lane 2-3 are amplification of CYP3A4*8, CYP3A4*1B and CYP3A4*15 alleles respectively and (a) for wild type and (b) for mutant type. Lane 2 had shown heterozygous which produce specific band for wild and mutant type for SNP CYP3A4*1B. This corresponds with sequencing chromatogram in Figure 1.2. The chromatogram had shown two peaks at position 262 which indicates heterozygous for sample in lane 2.
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