Set 1 Conc. (pmol) Set 3 Conc. (pmol) Set 5 Conc. (pmol) Pro Fw (5 pmol) 0.2 Pro Fw (5 pmol) 0.20 Ex12 Rv (5pmol) 0.15 Int6 Rv (5 pmol) 0.2 Ex11 Rv (5 pmol) 0.25 Ex5 Fw (5pmol) 0.3 CYP3A4*1B Wt/ CYP3A4*1B Mt  (5 pmol) 0.15 CYP3A4*14 Wt/ CYP3A4*14 Mt  (5 pmol) 0.15 CYP3A4*3 Wt/ CYP3A4*3 Mt  (5pmol) 0.15 CYP3A4*15 Wt/ CYP3A4*15 Mt  (5 pmol) 0.2 CYP3A4*11 Wt/ CYP3A4*11 Mt  (5 pmol) 0.30 CYP3A4*4 Wt/ CYP3A4*4 Mt  (5pmol) 0.3 CYP3A4*8 Wt/ CYP3A4*8 Mt  (5 pmol) 0.3 Set 4 Conc. (pmol)     Set 2 Conc. (pmol) Ex3 Rv (5 pmol) 0.4     Ex7 Fw (5 pmol) 0.3 Ex9 Rv (5 pmol) 0.4     Ex11 Rv (5 pmol) 0.25 Int6 Rv (5 pmol) 0.3     Ex5 Fw (5 pmol) 0.4 Ex11 Rv (5 pmol) 0.2     CYP3A4*9 Wt/ CYP3A4*9 Mt   (5 pmol) 0.4 CYP3A4*7 Wt/ CYP3A4*7 Mt  (5 pmol) 0.25     CYP3A4*5 Wt/ CYP3A4*5 Mt  (5 pmol) 0.25 CYP3A4*6 Wt/ CYP3A4*6 Mt  (5 pmol) 0.25     CYP3A4*16 Wt/ CYP3A4*16 Mt  (5 pmol) 0.1 CYP3A4*12 Wt/ CYP3A4*12 Mt  (5 pmol) 0.2     CYP3A4*13 Wt/ CYP3A4*13 Mt  (5 pmol) 0.3 CYP3A4*10 Wt/ CYP3A4*10 Mt  (5 pmol) 0.3

Table 3: Final concentration of each primers used for each Set in allele-specific second PCR.

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