Forward Reverse Product size (bp) First PCR Set A ProFw ProRv 831   Ex5Fw Int6Rv 757 Set B Ex3Fw Ex3Rv 360   Ex7Fw Ex7Rv 447   Ex9Fw Ex9Rv 412   Ex11Fw Ex11Rv 276   Ex12Fw Ex12Rv 173 Allele-specific second PCR Set 1 CYP3A4*8 Wt/ CYP3A4*8 Mt Int6Rv 516   ProFw CYP3A4*1B Wt/ CYP3A4*1B Mt 310   CYP3A4*15 Wt/ CYP3A4*15 Wt Int6Rv 153 Set 2 Ex5Fw CYP3A4*9 Wt/ CYP3A4*5 Mt 663   Ex7Fw CYP3A4*5 Wt/ CYP3A4*5 Mt 221   Ex7Fw CYP3A4*16 Wt/ CYP3A4*16 Wt 120   CYP3A4*13 Wt/ CYP3A4*13 Wt Ex11Rv 73 Set 3 ProFw CYP3A4*14 Wt/ CYP3A4*14 Wt 742   CYP3A4*11 Wt/ CYP3A4*11 Wt Ex11Rv 234 Set 4 CYP3A4*7 Wt/ CYP3A4*7 Wt Ex3Rv 261   CYP3A4*12 Wt/ CYP3A4*12 Wt Ex11Rv 206   CYP3A4*6 Wt/ CYP3A4*6 Wt Ex9Rv 178   CYP3A4*10 Wt/ CYP3A4*10 Wt Int6Rv 117 Set 5 Ex5Fw CYP3A4*4 Wt/ CYP3A4*4 Wt 242   CYP3A4*3 Wt/ CYP3A4*3 Wt Ex12Rv 101

Table 4: Primer combinations for amplification of first and allele-specific second PCR of CYP3A4.

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