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Figure 1c: Primer/probe set 1 and set 3 are the most sensitive sets to 97GH-AG2 viral RNA.
Viral RNA of 97GH-AG2 was subjected to real-time RT-PCR with a series of primer/probe sets to determine the efficiency of the different primers sets. The amount of total RNA (including carrier RNA and viral RNA) was 100 ng for each reaction.
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