| HIV RNA Assay Operators |
Sample Tested |
With-in Variation (Copies/ML) |
Between- run Variation (Copies/ML) |
| Run 1 |
Run 2 |
Run 3 |
Mean |
SDb |
CV (%)c |
| Operator 1 |
1 |
49 317 ± 165 |
51 881 ± 1321 |
45 640 ± 261 |
48 933.57 |
3137.71 |
6.41 |
| 2 |
26 121 ± 656 |
25 177 ± 1037 |
24 266 ± 867 |
25 187.96 |
785.81 |
3.12 |
| 3 |
8092 ± 162 |
7831 ± 125 |
8194 ± 139 |
8039.00 |
168.31 |
2.12 |
| Operator 2 |
1 |
50227 ± 712 |
53 718 ± 604 |
55706 ± 552 |
53217.00 |
2750.25 |
5.20 |
| 2 |
25821 ± 892 |
25 970 ± 986 |
27980 ± 1012 |
26590.00 |
1069.86 |
4.02 |
| 3 |
8235 ± 205 |
8521 ± 161 |
8408 ± 198 |
8388.00 |
94.70 |
1.12 |
aAll samples were tested in triplicate for each run. Every run was operated by different operator on 3 independent occasions.
bBetween-assay SDs were calculated using the modified SD formula: [(SD of means)2-(mean with-assay SD)2/3]1/2
cThe SD achieved was used to calculate the CV by using the formula CV=(SD/mean)×100 |
