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Figure 6: Distribution of HIV-p24 antigen levels between viral supernatant and magnetic beads. CCR5-tropic JFRL-pseudotyped HIV-1 was preincubated with peptidoliposomes and target cells were infected as described. CCR5- Beads – peptidoliposomes containing 5% each of NT-3FSN-CCR5-PAL and ECL2-CCR5-2PAL; (CD4+CCR5)-Beads – peptidoliposomes containing 5% each of CD4M48P1-PAL, ECL2-CCR5-2PAL and NT-3FSN-CCR5-PAL; solCD4 – CD4M48 peptide (10 μM). Magnetic liposomes displaying no peptides (Empty Beads) were used as the control (set to 100 %). At the end of the incubation, the supernatant and liposome fractions were analyzed by ELISA for the presence of HIV-p24 antigen as described in the Experimental Procedures. The data are mean ± S.E.M calculated from three independent experiments performed in duplicates.
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