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| Figure 2: PPS-specific B cells from HIV-positive individuals immunized with PPV23. HIV-positive donors were immunized with PPV23. On day 0 and day 7 post-immunization, lymphocyte enriched peripheral blood samples stained with fluorescently labeled PPS14 or PPS23F were evaluated by flow cytometry for distribution of CD27 and IgM among PPS-selected CD19+ B cells. Results for all HIV-positive individuals were categorized by CD4 counts and HAART usage. B cells were expressed as a percentage of total unselected or PPS-selected CD19+ B cells (A,B). B cells were expressed as the number of PPS-selected CD19+ B cells per ml (C,D). In each sample, 100,000 events were recorded. Data are mean ± standard error of the mean. *p< 0.05, **p< 0.01, ***p< 0.001. |
