| A. Switched Memory B cell subset distribution (cells/ml) |
| Patient groups |
PPS14- (Unselected) Day 0 |
PPS14+ (Selected) Day 7 |
PPS23F- (Unselected) Day 0 |
PPS23F+ (Selected) Day 7 |
| Group A (CD4>200) |
624±200 |
1022±155 |
525±104 |
1215±196** |
| Group B (CD4<200) |
159±48 |
731±212* |
162±28 |
1653±467* |
| B. IgM Memory B cell subset distribution (cells/ml) |
| Patient groups |
PPS14- (Unselected) Day 0 |
PPS14+ (Selected) Day 7 |
PPS23F- (Unselected) Day 0 |
PPS23F+ (Selected) Day 7 |
| Group A (CD4>200) |
317±60 |
1109±142*** |
331±47 |
1458±233*** |
| Group B (CD4<200) |
184±56 |
1281±368* |
239±64 |
1653±467** |
| C. Naive B cell subset distribution (cells/ml) |
| Patient groups |
PPS14- (Unselected) Day 0 |
PPS14+ (Selected) Day 7 |
PPS23F- (Unselected) Day 0 |
PPS23F+ (Selected) Day 7 |
| Group A (CD4>200) |
1116±326 |
1503±235
|
524±101 |
1212±158*** |
| Group B (CD4<200) |
675±220 |
555±135 |
635±205 |
907±250 |
Data are mean ± standard error of the mean. * indicates significant rise in the post vaccination (day7) levels compared to pre-vaccination (day 0) within a group. Cell counts
of specific cell population in donor groups are reported as the average value of = [(percentage of specific cell population) * total lymphocytes/ml] for that group. Pre to post
vaccination changes in a single group were compared using paired t-test and between groups were compared using analysis of co-variance (ANCOVA).*p<0.05, **p<0.01,
***p<0.001.