Condition Values evaluated Polymerase enzyme        0.5U; 1.0U; 1.5U; 2.0U   Primers        0.2µm; 0.3µm; 0.4µm; 0.5µm Magnesium chloride Annealingtemperature        1.0mM; 2.0mM; 3.0mM; 4.0mM57°C; 58.6°C;  60.1°C;  61.9°C; 63.4°C

Table 1: PCR conditions evaluated to optimize the multiplex PCR assay.

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