Protocol Optimal concentrations Amplification conditions  MB1-529 Primers B22-B23: 0.5 µM   95°C 3 min Primers 529F-529R: 0 5 µM 35 cycles of  (95°C  30 seconds, 60.1°C  1 min, 72°C  1 min) MgCl2: 2.0 mM 72°C  5 min Taq DNA polimerase:      1U 4°C

Table 3: Optimal concentrations of reagents and amplification conditions for multiplex PCR assay (MB1-529).

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