Antimicrobial
Figure 4: Antimicrobial peptides inhibit TLR4, TLR1/2 and CVL induced proinflammatory response in vitro.(A-C) THP-1 cells were pre-stimulated with different antimicrobial peptides (10ug/ml), either alone or in combinations for 1 hour followed by LPS (200 ng/ml) orPAM3csk (100 ng/ml) treatment for 12 hours. TNF-alpha and IL-6 were measured in the culture supernatants by ELISA. Data represent mean +/- SD of two independentexperiments. (D) Relative expressions of IFN beta mRNA (GAPDH normalized) was measured by RT-qPCR after 3 hrs of LPS treatment (mean +/- SD valuesrepresentatives of two independent experiments). Significant differences comparing with or without addition of antimicrobial peptide were assessed by nonparametricMann-Whitney U test and are indicated by *P< 0.05, **P <0.01, and***P <0.001. (E) THP 1 cell-derived macrophages were stimulated with CVLs from WCR (HIV+) andWDR( HIV-) women (10% of culture volume) for 24 hrs in the presence of gentamycin (50ug/ml). TNF-alpha were measured in the culture supernatants by ELISA andanalysed by nonparametric Mann-Whitney U test: (WCR=67, WDR=26). (F, G) THP-1 cells were pre-stimulated with hBD3 (10 ug/ml), for 1 hour followed by CVLs from either WCR (F) or WDR (G) individuals; P value was calculated by the Wilcoxon matched-pairs test.
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