Mice were immunized with wild type gp120 (wt) or immune complexes containing
wild type gp120LAI (wt) and anti-CD4-binding site mAb 654-D. Immunization
was performed by intraperitoneal injections, 3 times at 2-week intervals, with
MPL/DDA adjuvant [55]. Immune complexes made of mutant gp120 (N448Q or
N448E) and mAb 654-D were also tested [55]. In addition, three groups of mice
received intramuscular injections of DNA plasmids encoding wild type or mutant
gp120 followed with subcutaneous boosts with the corresponding gp120
proteins in QS21 adjuvant (DNA/P) [55]. Two weeks after the last immunization,
serum neutralizing activities were measured against pseudotyped HIV-1
bearing the homologous LAI Env in TZM-bl target cells. The bars in each group
represent data from serum serially diluted three fold starting from 1:50. Means
and standard deviations from duplicate wells are shown.