Immunological parameters of V3 epitopes

Immunogen	Neutralizing Ab activity^a	Antigenicity^b	Proteolytic degradation^c	Helper T cell response^d
wt	-	+	+	++
N448Q	-	++	++	+++
wt/654-D	+	++	++	+
N448Q/654-D	++	+++	+	+

^aNeutralizing Ab activity against the homologous LAI virus was determined based on reduction of virus infection by immune sera at 1:50 dilution in standard TZM-bl assays. (-) 0-50%; (+) 50-75%; (++) 75-100% [55,56].
^bV3 antigenicity on the respective immunogens based on recognition by neutralizing anti-V3 mAbs [55,56].
^cRelative sensitivity of V3 epitopes to cathepsin L digestion [55].
^dgp120-specific T cell proliferative responses of immunized animals measured in 3H-thymidine incorporation assays [55,66].

Table 1: Immunological parameters of V3 epitopes presented on wild type or mutant gp120 on its own or as immune complexes with mAb 654-D.

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