Immunogen

Neutralizing
Ab activitya

Antigenicityb

Proteolytic degradationc

Helper T cell responsed

wt

-

+

+

++

N448Q

-

++

++

+++

wt/654-D

+

++

++

+

N448Q/654-D

++

+++

+

+

aNeutralizing Ab activity against the homologous LAI virus was determined based on reduction of virus infection by immune sera at 1:50 dilution in standard TZM-bl assays. (-) 0-50%; (+) 50-75%; (++) 75-100% [55,56].
bV3 antigenicity on the respective immunogens based on recognition by neutralizing anti-V3 mAbs [55,56].
cRelative sensitivity of V3 epitopes to cathepsin L digestion [55].
dgp120-specific T cell proliferative responses of immunized animals measured in 3H-thymidine incorporation assays [55,66].
Table 1: Immunological parameters of V3 epitopes presented on wild type or mutant gp120 on its own or as immune complexes with mAb 654-D.
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