The flow of steps in the process is shown here. The original complex antigen or a subcomponent of it is co-crystallized with a bNMAb to reveal atomic-level structural information about the epitope. The epitope is then recapitulated as a constrained mimic using scaffolds as backbones to present the epitope. If several different backbones are generated that present essentially the same epitopic structure, then these can be used in a series of heterologous immunizations to selectively amplify B cell responses to the epitope. If the strategy works, then the outcome would be the generation of a polyclonal B cell response containing bNMAb specificities that resemble the original template bNMAb.
