Group   N GAPDH –Ct
(Ct-G)
IL-25-Ct
(Ct-25)
2∆∆Ct
OVA 10 15.79 ± 0.81 16.03 ± 5.87a 1.48 ± 0.07a
control 10 14.02 ± 0.57 29.65 ± 0.74 0.74 ± 0.03
GC 10 14.21 ± 0.69 27.48 ± 1.28b 0.81 ± 0.06b
F-value   42.75 59.82 105.34
P-value   <0.01 <0.01 <0.01
aIndicates a p-value of less than 0.05, compared with control group
bIndicates a p-value of less than 0.05, compared with OVA group
Table 3: Quantitative detection of IL-25 mRNA in airway epithelium image.
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