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Figure 2: Western blot analysis showing the IgE reactivity of major protein bands from house dust mite aqueous extracts using five allergic patients’ sera. A: Blomia tropicalis extract; B: Dermatophagoides farina extract; and C: Dermatophagoides pteronyssinus extract. Lane 1: protein marker (BioRad Pre-stained Standards Broad Range) while lanes 2-6: different sera from selected allergic patients. Arrow A: majority of patient’s IgE reacted to ~70kDa protein; Arrow B: majority of patient’s IgE reacted to ~80kDa protein; Arrow C: majority of patient’s IgE reacted to ~60kDa protein.
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