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Figure 1: Dot plots illustrating the gating strategy used for the immunophenotypic identification and characterization of basophils (Panel A) and T cells (Panel B) in peripheral blood samples. Panel A: Basophils (black events) were selected as SSC (low) CD45+dim (A1) and CD123+bright (A2) events and analyzed for the expression of CD123 and CD294 (A3). Panel B: T cells (black events) were selected as SSC (low), CD3+ events (B1) and analyzed for the expression of CD294 (CRTH2) (B2) and HLA-DR (B3). Other peripheral blood leukocytes are shown as grey events.
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