Pacific orange conjugated anti-CD45, phycoerythrin Cy-7 conjugated anti- CD123, and phycoerythrin conjugated anti-CD294 were used in combination with other monoclonal antibodies.
Median fluorescence intensity is expressed as arbitrary units scaled from 0 to 100,000.
Boxes extend from the 25th to the 75th percentiles; the line in the middle and the vertical lines represent median values and 95% confidence intervals, respectively. No significant differences (P>0.05) were observed between the median fluorescence intensity of CD45, CD123 and CD294 (CRTH2) expression on peripheral blood basophils from patients and controls using the Mann-Whitney U-test.
