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| Figure 5: Nitrite levels in the BAL and iNOS expression in lung tissue of WT and cav-1-/- mice BAL and lung tissue were collected 4 hours after LPS aerosol exposure (cav- 1-/- LPS and WT LPS). cav-1-/- (cav-1-/- sham) and WT (WT sham) mice were exposed to PBS as a sham treatment. A) Nitrite levels in the BAL were assessed by the Greiss assay. Data is reported as fold change over WT sham. (*, p < 0.05, n = 11 - 14) Total RNA was extracted from 1 lung lobe and B) iNOS expression was assessed using quantitative real-time PCR analysis. The data were normalized to the WT sham group within each individual experiment before being combined (**, p < 0.01, n = 7 - 14). C) Lung tissue homogenate from WT sham(*,p<0.05, n = 11-14) treated mice was subjected to density separation on a sucrose gradient and the resulting fractions were immunoblotted for iNOS and cav-1. A representative blot is shown. |
