Primer Sequence Melting temperature (Tm) Product size (bp) Reference gene   β-actin Forward TGGGTATGGAATCCTGTGG 55.78 96 Reverse GCACTGTGTTGGCATAGAGG 56.18 Target genes:   iNOS Forward GGAGCCTTTAGACCTCAACAGA 58.03 104 Reverse GGCTGGACTTTTCACTCTGC 57.17 TNF-α Forward CCACCACGCTCTTCTGTC 57.79 114 Reverse ATCTGAGTGTGAGGGTCTGG 57.62 TGF-β1 Forward GCTAATGGTGGACCGCAAC 58.57 100 Reverse CACTGCTTCCCGAATGTCTG 58.72

Table 1: Primers used in real-time polymerase chain reaction.

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