(A) Macroscopic changes in tissue sites generated by the injection of 70% ethanol
(E) and 70% ethanol/0.1% dexamethasone (E/Dex) were observed at various
time points. The injection of saline (Sa) gave temporary vesicular swelling,
but such swelling disappeared within 5h, and on day 1 the Sa-injected sites
showed no significant difference from the sites that were not injected. (B) The
injured area of E- and E/Dex-injected sites (filled and open bars, respectively)
at each time point were analyzed using Image J software. The data represent
means ± standard deviation (SD; n = 7 for 5 h, and 1, 3, and 5 d; n = 3 for 7
and 14 d). No data was obtained for the Sa-injected site, since the injured area
was hardly recognized even at 5 h. (C) The dorsal skin sections were prepared
at each time point after injection and stained with hematoxylin and eosin (HE),
and Schematic diagram represents the injured areas after injection of E or E/S.
Bars: 1 mm. *P < 0.05. |
