Target gene

Forward primer

Reverse primer

Cxcl1

5¢-GCTTGAAGGTGTTGCCCTCAG-3′

5′-AAGCCTCGCGACCATTCTTG-3′

Cxcl2

5′-GCGCTGTCAATGCCTGAAGA-3′

5′-TTTGACCGCCCTTGAGAGTG-3′

Il-1b

5′-TCCAGGATGAGGACATGAGCAC-3′

5′-GAACGTCACACACCAGCAGGTTA-3′

Il-6

5′-CCACTTCACAAGTCGGAGGCTTA-3′

5′-GCAAGTGCATCATCGTTGTTCATAC-3′

Spp1 (Opn)

5′-TACGACCATGAGATTGGCAGTGA-3′

5′-TATAGGATCTGGGTGCAGGCTGTAA-3′

Has1

5′-TGTGTGTCCTGCATCAGTGGTC-3′

5′-TGGTTGTACCAGGCCTCCAAG-3′

Has2

5′-AGTCATGTACACAGCCTTCAGAGCA-3′

5′-GGCAGGGTCAAGCATAGTATCTGAG-3′

Has3

5′-CATTGAGATGCTTCGAGTCTTGGA-3′

5′-TACATTGCACACAGCCAAAGTAGGA-3′

Vcan

5′-ATGGGATTGAAGACACTCAGGACAC-3′

5′-TGAGCAGCGGCAAAGTTCAG-3′

Gapdh

5′-TGTGTCCGTCGTGGATCTGA-3′

5′-TTGCTGTTGAAGTCGCAGGAG-3′

Has: Hyaluronan synthase; Il: Interleukin; Gapdh: Glyceraldehyde 3-phosphate Dehydrogenase; Opn: Osteopontin; Vcan: Versican
Table 2: Primer sequences for real-time reverse transcriptase polymerase chain reaction.
Goto home»