image
Figure 1: Characterization of the 37kDa biologically active form of Anx- A1 in healthy controls and the 33kDa proteolytically cleaved form of Anx-A1 in VKC patients
(A) Representative blot of tear specimens collected from healthy donors, non-treated patients with VKC and Lodoxomide treated VKC individuals. Lactoferrin was used as positive control in our assay since its constitutive expression in tear film. Anx-A1 37kDa is highly expressed in control group, while a 33kDa band appears in VKC tear specimens and interestingly abrogated in patients treated with Lodoxomide. (B) Densitometry of n=17 control group, n=23 VKC group and n=12 Lodoxomide treated group showing a significantincreasein the amount of the cleaved (inactive) form of Anx-A1 in VKC patients compare to controls (p<0.01). This cleavage is protected in patients treated with Lodoxomide.
Goto home»