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Figure 5: E‾/B48 lipoproteins selectively activated PERK, but not PKR or GCN2. Raw 264.7 cells were incubated at 37C for 12 hrs with 20 g/ml of E+/B48 or E‾/B48 lipoproteins or culture medium alone (control). The total protein levels of PERK, PKR, and GCN2, and the phosphorylated forms of PERK (PERK-p), PKR (PKR-p), and GCN2 (GCN2-p) were determined by western blot analysis and expressed relative to -tubulin protein levels. Values represent the mean SEM of five separate experiments. *P<0.05 as compared with the control.
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